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Related Experiment Video

Updated: Aug 29, 2025

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Inferring cell communication using single-cell calcium spatiotemporal dynamics.

Nika Taghdiri¹, Kevin R King²

¹Department of Bioengineering, Jacobs School of Engineering, University of California San Diego, La Jolla, CA, USA.

|September 6, 2022

Summary

This summary is machine-generated.

This study introduces a new protocol using genetically encoded calcium indicator (GECI) mice to detect cell communication events. The method analyzes calcium fluorescence signals for inferring cell signaling in live imaging and microscopy.

Keywords:

Bioinformatics Cell biology Microscopy Model organisms Molecular biology Signal transduction

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Last Updated: Aug 29, 2025

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Imaging Local Ca2+ Signals in Cultured Mammalian Cells

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Published on: March 3, 2015

Area of Science:

Neuroscience
Cell Biology
Biophysics

Background:

Limited experimental tools exist for non-destructive cell communication discovery.
Genetically encoded calcium indicators (GECI) offer potential for monitoring cellular activity.

Purpose of the Study:

To describe a protocol for analyzing GECI fluorescence data.
To enable the detection of cell communication events in vitro and in vivo.

Main Methods:

Utilizing tissue-specific GECI mice.
Preprocessing GECI fluorescence time-series data from live cell imaging or intravital microscopy.
Detecting single-cell calcium fluorescence transients and analyzing peak synchrony.

Main Results:

A protocol for preprocessing and analyzing GECI fluorescence data was established.
The method allows for the inference of putative cell communication events based on synchronized calcium transients.
Successful application in live cell imaging and intravital microscopy settings.

Conclusions:

The developed protocol provides a valuable tool for studying cell communication non-destructively.
This method enhances the ability to investigate intercellular signaling dynamics in biological systems.
Further research can build upon this protocol for advanced cell communication studies.