Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Southern Blot02:57

Southern Blot

20.0K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
20.0K
Labeling DNA Probes03:31

Labeling DNA Probes

8.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential Roles of BRCA1 and BRCA2 in the DNA Damage Response Revealed by isogenic V79 Mutant Cell lines.

Mutagenesis·2026
Same author

Generation of NBS1 knockout in Chinese hamster cells revealed ATR role for radiation and etoposide induced DNA damage in absence of NBS1 proteins.

Frontiers in oncology·2026
Same author

Biological effectiveness of superficial X-ray in mammalian cells through precise dosimetry and Monte Carlo simulations.

Biochemical and biophysical research communications·2026
Same author

Dr. Bradford D. Loucas (1957-2025).

Radiation research·2025
Same author

A novel radiosensitizer α-sulfoquinovosyl-acylpropanediol (SQAP) inhibits DNA repair pathways and sensitize cells to cancer chemotherapeutic agents.

Scientific reports·2025
Same author

DDR kinase inhibition causes hypersensitivity to Taxol through caspase-3 activation.

Biochemical and biophysical research communications·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 29, 2025

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans
10:01

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

Published on: August 4, 2016

10.5K

Nontraditional Method for Telomere Staining by PNA Probes.

Takamitsu A Kato1

  • 1Department of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA. Takamitsu.Kato@colostate.edu.

Methods in Molecular Biology (Clifton, N.J.)
|September 6, 2022
PubMed
Summary
This summary is machine-generated.

This study presents a novel PNA telomere staining protocol that bypasses DNA-DNA hybridization. This non-traditional method offers a faster way to visualize telomere locations on metaphase chromosomes.

Keywords:
Non-nucleic acid hybridization methodNucleoprotein complexPNA probesTelomere

More Related Videos

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

46.7K
Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
09:07

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Published on: March 4, 2021

3.0K

Related Experiment Videos

Last Updated: Aug 29, 2025

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans
10:01

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

Published on: August 4, 2016

10.5K
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

46.7K
Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci
09:07

Fluorescence In Situ Hybridization on DNA Halo Preparations to Reveal Whole Chromosomes, Telomeres and Gene Loci

Published on: March 4, 2021

3.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Standard Fluorescence In Situ Hybridization (FISH) relies on DNA-DNA hybridization, which can be time-consuming.
  • Proteins binding to specific DNA sequences regulate gene function.
  • Telomere-binding proteins protect chromosome ends by forming nucleoprotein complexes.

Purpose of the Study:

  • To introduce a nontraditional protocol for telomere staining.
  • To visualize telomere locations on metaphase chromosomes without DNA-DNA hybridization.

Main Methods:

  • Utilizing peptide nucleic acid (PNA) probes for telomere detection.
  • Employing a non-hybridization manner for probe accumulation at telomere sites.

Main Results:

  • Demonstration of a PNA telomere staining protocol.
  • Successful visualization of telomere locations on metaphase chromosomes.

Conclusions:

  • The PNA telomere staining protocol offers an alternative to traditional FISH.
  • This method allows for efficient visualization of telomeres without DNA-DNA hybridization.