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Live Cell Synthetic Histone Acetylation by Chemical Catalyst
Shigehiro A Kawashima1, Motomu Kanai2
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan. skawashima0418@gmail.com.
Abstract:
Posttranslational modifications (PTMs) of histones, such as lysine acetylation and ubiquitination, regulate chromatin structure and gene expression. In living organisms, histone PTMs are catalyzed by histone-modifying enzymes. Here, we describe an entirely chemical method to introduce histone modifications in living cells without genetic manipulation. The chemical catalyst PEG-LANA-DSSMe activates a thioester acetyl donor, N,S-diacetylcysteamine (NAC-Ac), and promotes regioselective, synthetic histone acetylation at H2BK120 in living cells.
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