Scalable Nanotrap Matrix Enhanced Electroporation for Intracellular Recording of Action Potential

Electrophysiological recording, as a long-sought objective, plays a crucial role in fundamental biomedical research and practical clinical applications. The challenge in developing electrophysiological detection platforms is to combine simplicity, stability, and sensitivity in the same device. In this study, we develop a nanotrapped microelectrode based on a porous PET membrane, which is compatible with large-scale microtechnologies. The nanotraps can promote the protrusion of the local cell membrane in the hollow center and offer a unique nanoedge structure for tight sealing and effective electroporation. We demonstrate that scalable nanotraps can enhance cell-electrode coupling and perform high-quality intracellular recording. Further, the nanoedge-enhanced electroporation and minimally invasive nanotrapped recordings afford much longer intracellular access of over 100 min and permit consecutive electroporation events in a short period of time. This study suggests that the geometry-regulating strategy of the cell-electrode nanointerface could significantly improve the intracellular recording performance of a nanopatterned electrode.