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Core-shell bioprinting of vascularizedin vitroliver sinusoid models
Rania Taymour1, Nathaly Alejandra Chicaiza-Cabezas1, Michael Gelinsky1
1Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
Biofabrication
|September 7, 2022
Summary
This study developed a 3D bioprinted liver model using coaxial extrusion. The core-shell structure mimics liver sinusoids, enhancing cell interactions and albumin secretion for better disease modeling.
Area of Science:
- Tissue Engineering
- Biomaterials Science
- Cell Biology
Background:
- In vitro liver models are crucial for studying cell behavior and microenvironmental responses.
- Mimicking the complex three-dimensional (3D) microarchitecture is a key challenge in liver tissue engineering.
Purpose of the Study:
- To develop a liver sinusoid-like model using coaxial extrusion-based 3D bioprinting.
- To investigate cell-cell interactions and functionality in a complex engineered tissue.
Main Methods:
- Coaxial extrusion 3D bioprinting of a core-shell liver model.
- Shell ink: alginate-methylcellulose (algMC) in plasma with HepG2 cells.
- Core ink: collagen-fibrin-gelatin (CFG) with endothelial cells and fibroblasts.
Main Results:
- The algMC blend provided printing fidelity and stability.
- Plasma enhanced HepG2 viability, cluster formation, and biomarker expression.
- The core ink supported pre-vascular network formation.
- Triple co-culture enhanced albumin secretion.
Conclusions:
- Core-shell bioprinting is effective for creating complex, tissue-like liver models.
- This model facilitates the study of cell-cell interactions in a 3D environment.
- The developed model shows potential for disease investigation and drug testing.
