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Development of aptamers for rapid airborne bacteria detection
In Hwa Jeong1,2, Ho Kyeong Kim1,2, Hye Ri Kim2,3
1Department of Chemical and Biological Engineering, Korea University, Seoul, Korea.
Analytical and Bioanalytical Chemistry
|September 7, 2022
Summary
Researchers developed a rapid aptamer isolation method to detect airborne Citrobacter braakii (C. braakii). The CB-5 aptamer demonstrates high affinity and specificity for this airborne pathogen, enabling fast and sensitive diagnostics.
Area of Science:
- Microbiology
- Biotechnology
- Molecular Diagnostics
Background:
- Airborne microbes pose global infectious disease risks.
- Rapid and sensitive detection methods for airborne pathogens are crucial.
- No specific receptors for airborne Citrobacter braakii (C. braakii) have been previously identified.
Purpose of the Study:
- To rapidly isolate and characterize aptamers that specifically bind to the airborne bacterium C. braakii.
- To establish a novel protocol for developing diagnostic receptors against airborne pathogens.
Main Methods:
- Isolation of aptamers targeting C. braakii using a centrifugation-based partitioning method (CBPM).
- Assessment of aptamer binding affinity and specificity using bacteria in liquid culture and aerosolized samples.
- Characterization of aptamer performance in a simulated airborne environment.
Main Results:
- The CB-5 aptamer was rapidly isolated with high affinity and specificity for C. braakii.
- Binding affinity (Kd) was measured as 16.42 nM in liquid culture and 26.91 nM in aerosolized samples.
- The CBPM protocol proved effective for developing diagnostic receptors for airborne bacteria.
Conclusions:
- The developed CBPM facilitates the rapid isolation of specific aptamers for airborne pathogens.
- The CB-5 aptamer is a promising candidate for developing sensitive diagnostic tools for C. braakii.
- This approach holds potential for the rapid development of diagnostics for various airborne bacterial threats.
