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A CRISPR-based lateral flow assay for plant genotyping and pathogen diagnostics.
Edith Sánchez1, Zahir Ali1, Tofazzal Islam2
1Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Plant Biotechnology Journal
|September 8, 2022
Summary
A new field-deployable assay, Bio-SCAN, rapidly identifies elite crop germplasm and detects pathogens. This tool enhances crop breeding, genetic engineering, and disease diagnostics for improved food security.
Area of Science:
- Agricultural Science
- Molecular Biology
- Plant Pathology
Background:
- Current crop germplasm selection and pathogen detection methods are inefficient, costly, and difficult to scale for field applications.
- Developing rapid, sensitive, and field-deployable diagnostic tools is crucial for effective disease management and crop improvement.
Purpose of the Study:
- To optimize and validate Bio-SCAN, a field-deployable lateral flow assay for rapid germplasm identification and pathogen detection.
- To demonstrate the versatility of Bio-SCAN in various agricultural applications, including genotyping, transgene detection, and phytopathogen screening.
Main Methods:
- Optimization of a lateral flow assay (Bio-SCAN) for in-field use.
- Application of Bio-SCAN for genotyping wheat germplasm for disease resistance alleles (Lr34, Lr67).
- Detection of herbicide-resistant alleles in rice and transgenic elements in tobacco and rice.
- Screening for viral, bacterial, and fungal pathogens in plants.
Main Results:
- Bio-SCAN achieved results in under 1 hour with high sensitivity and 100% specificity for transgene detection.
- Successfully genotyped wheat for disease resistance alleles and detected specific pathogens like Puccinia striiformis and Magnaporthe oryzae.
- Demonstrated effective detection of viral and bacterial pathogens in Nicotiana benthamiana.
Conclusions:
- Bio-SCAN is a highly sensitive, simple, versatile, and field-deployable molecular diagnostic tool.
- The assay significantly enhances capabilities in crop breeding, genetic engineering, and pathogen diagnostics.
- Bio-SCAN holds great potential for improving crop productivity and ensuring food security.
