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Related Concept Videos

X-ray Crystallography02:18

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The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
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Related Experiment Video

Updated: Aug 29, 2025

Fixed Target Serial Data Collection at Diamond Light Source
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Fixed-target serial crystallography at the Structural Biology Center.

Darren A Sherrell1, Alex Lavens1, Mateusz Wilamowski2

  • 1Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Lemont, IL 60439, USA.

Journal of Synchrotron Radiation
|September 8, 2022
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Summary
This summary is machine-generated.

A new 3D-printed mesh-holder enables serial synchrotron crystallography by immobilizing crystal samples. This method allows for rapid data collection and analysis of protein structures, including key beta-lactamases.

Keywords:
X-ray free-electron lasersfixed-target serial synchrotron crystallographystructural biology

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Area of Science:

  • Structural biology
  • Biophysics
  • Crystallography

Background:

  • Serial synchrotron crystallography (SSC) is crucial for studying protein structures under physiological conditions.
  • Existing SSC methods face challenges with sample handling and radiation damage.
  • Advancements are needed for efficient data collection from numerous crystals.

Purpose of the Study:

  • To introduce a novel 3D-printed mesh-holder for SSC.
  • To optimize sample immobilization and handling for X-ray crystallography.
  • To apply the system to study important beta-lactamase enzymes.

Main Methods:

  • Development of a 3D-printed mesh-holder for immobilizing crystal suspensions.
  • Utilizing a fixed-target approach with Mylar films for crystal trapping.
  • Employing piezoelectric stages and a high-performance computing pipeline for data acquisition and analysis.

Main Results:

  • The new holder effectively immobilizes near-monolayers of crystals in their mother liquor.
  • Rapid data collection in scan mode was achieved.
  • The system was successfully applied to determine structures of two beta-lactamases.

Conclusions:

  • The 3D-printed mesh-holder system enhances serial synchrotron crystallography efficiency.
  • This method facilitates near real-time analysis of protein structures.
  • The system is applicable to diverse protein targets, including clinically relevant enzymes.