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Related Experiment Video

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Casting Zinc Oxide Nanoparticles Using Fagonia Blend Microbial Arrest.

Riaz Hussain1, Ayesha Zafar1,2, Murtaza Hasan3,4

  • 1Department of Zoology, Kohat University of Science and Technology, Kohat, 26000, Pakistan.

Applied Biochemistry and Biotechnology
|September 8, 2022
PubMed
Summary

This study synthesized zinc oxide nanoparticles (ZnO NPs) using Fagonia cretica plant extract, demonstrating eco-friendly production. The green-synthesized ZnO NPs exhibited significant antibacterial and antioxidant properties, paving the way for medical and industrial applications.

Keywords:
Antimicrobial activityBioreducing agentGreen synthesisPlant extractZinc NPs

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Area of Science:

  • Green Chemistry
  • Nanotechnology
  • Materials Science

Background:

  • Conventional physical and chemical nanoparticle synthesis methods pose environmental and toxicological risks.
  • There is a growing need for sustainable and biocompatible nanoparticle production techniques.
  • Plant-derived compounds offer a promising alternative for eco-friendly synthesis.

Purpose of the Study:

  • To synthesize zinc oxide nanoparticles (ZnO NPs) using the aqueous extract of Fagonia cretica.
  • To characterize the synthesized ZnO NPs for their structural, morphological, and optical properties.
  • To evaluate the antibacterial and antioxidant activities of the green-synthesized ZnO NPs.

Main Methods:

  • Phytochemical screening of Fagonia cretica extract to identify bioactive compounds.
  • UV-Visible spectroscopy, X-ray Diffraction (XRD), and Transmission Electron Microscopy (TEM) for characterization.
  • Fourier Transform Infrared (FTIR) spectroscopy to determine the role of phytochemicals in stabilization.
  • Antibacterial assays against Staphylococcus aureus and Escherichia coli.
  • Antioxidant activity assessment using standard assays.

Main Results:

  • Fagonia cretica extract contains various bioactive compounds, including phenols and proteins, acting as stabilizing agents.
  • UV-Visible spectroscopy confirmed ZnO NPs with a peak at 362 nm.
  • XRD analysis revealed a crystalline wurtzite hexagonal structure for the ZnO NPs.
  • TEM imaging showed flower-shaped ZnO NPs ranging from 100-1000 nm.
  • The synthesized ZnO NPs demonstrated potent antibacterial activity against both Gram-positive and Gram-negative bacteria.
  • Significant antioxidant activity was observed, with percentages increasing with concentration.

Conclusions:

  • Fagonia cretica provides an effective and sustainable source for green synthesis of ZnO NPs.
  • The flower-shaped ZnO NPs possess notable antibacterial and antioxidant properties.
  • This eco-friendly approach offers a promising route for developing novel nanomaterials for medicinal and industrial applications.