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Related Experiment Video

Updated: Aug 29, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

Event-triggered STED imaging.

Jonatan Alvelid¹, Martina Damenti¹, Chiara Sgattoni¹

¹Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden.

|September 8, 2022

Summary

This summary is machine-generated.

Event-triggered STED microscopy automates nanoscale imaging of cellular events in real time. This advanced technique overcomes photobleaching limitations for unprecedented live cell dynamics observation.

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Published on: April 9, 2018

Related Experiment Videos

Last Updated: Aug 29, 2025

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

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Published on: March 24, 2014

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

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Published on: December 20, 2021

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion STED Microscopy

Published on: April 9, 2018

Area of Science:

Cellular biology
Microscopy
Biophysics

Background:

Cellular processes rely on proteins and lipids, necessitating high-resolution imaging.
Stimulated Emission Depletion (STED) nanoscopy offers fast live-cell imaging but suffers from photobleaching.

Purpose of the Study:

To develop an automated method for initiating STED imaging based on cellular events.
To enhance temporal resolution for observing dynamic cellular processes.

Main Methods:

Developed event-triggered STED, an automated multiscale imaging approach.
Utilized biosensors to detect cellular events like protein recruitment and vesicle trafficking.
Applied STED microscopy in proximity to detected events for maximized temporal resolution.

Main Results:

Successfully imaged synaptic vesicle dynamics at 24 Hz, 40 ms post-calcium activity.
Observed endocytosis/exocytosis events at 11 Hz, 40 ms post-protein recruitment or pH change.
Monitored endosomal vesicle interactions at 3 Hz, 70 ms post-approach.

Conclusions:

Event-triggered STED significantly advances live nanoscale imaging capabilities.
Enables real-time biological observations of dynamic cellular events.
Opens new avenues for studying cellular mechanisms with high spatiotemporal precision.