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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Aug 29, 2025

Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light

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Published on: August 17, 2017

Synchrotron Infrared Microspectroscopy for Stem Cell Research.

Jiang Qian^1,2,3,4, Xue Gao⁵, Ya-Di Wang^2,5

¹Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

International Journal of Molecular Sciences

|September 9, 2022

Summary

This summary is machine-generated.

Synchrotron infrared microspectroscopy offers a non-invasive method to study stem cell behavior. This technique analyzes biomolecule distribution, aiding in understanding stem cell differentiation and fate determination for regenerative medicine.

Keywords:

cancer stem cells pluripotent stem cells stem cell differentiation synchrotron infrared microspectroscopy

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Published on: December 17, 2015

Area of Science:

Biotechnology
Cell Biology
Spectroscopy

Background:

Stem cells are crucial for tissue repair due to self-renewal and differentiation.
Stem cell niches influence their behavior but are complex to study.
Current methods for stem cell analysis have limitations.

Purpose of the Study:

To review the potential of synchrotron infrared microspectroscopy (SR-FTIR) in stem cell research.
To highlight SR-FTIR's capability in analyzing stem cell differentiation and fate.
To explore SR-FTIR as a non-invasive tool for stem cell diagnostics.

Main Methods:

Synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIR) is utilized.
Non-invasive and non-biological probes are employed.
Quantitative mapping of biomolecule content and distribution is performed.

Main Results:

SR-FTIR provides unique vibration bands for molecular analysis.
The technique enables detailed characterization of biomolecules within cells and tissues.
Potential for precise monitoring of stem cell differentiation processes.

Conclusions:

SR-FTIR is a promising technology for advancing stem cell research.
It offers a powerful, non-invasive approach to investigate stem cell fate.
This method can aid in developing new diagnostic and therapeutic strategies.