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Updated: Aug 29, 2025

Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
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Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis.

Vuk Uskoković1,2, Victoria M Wu1

  • 1TardigradeNano LLC, Irvine, CA 92604, USA.

Materials (Basel, Switzerland)
|September 9, 2022
PubMed
Summary
This summary is machine-generated.

Hydroxyapatite (HAp) nanoparticles selectively reduced Gram-negative bacteria in soil microbiomes, increasing beneficial Gram-positive bacteria. This antibacterial property of HAp offers potential in environmental and biomedical applications.

Keywords:
16S ribosomal next-generation sequencingbacterial microbiomeenvironmental remediationmetagenomicsnanoparticles

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Area of Science:

  • Environmental Microbiology
  • Materials Science
  • Metagenomics

Background:

  • Hydroxyapatite (HAp), a biomineral, exhibits moderate antibacterial properties.
  • Metagenomics enables analysis of material-microbe interactions for optimizing antibacterial activity.
  • Understanding HAp's effect on complex microbial communities is crucial for its applications.

Purpose of the Study:

  • To investigate the impact of HAp nanoparticles on a soil microbiome.
  • To analyze the selective antibacterial activity of HAp against Gram-negative and Gram-positive bacteria.
  • To propose a model explaining the mechanism of HAp's selective binding.

Main Methods:

  • Challenging a soil microbiome with HAp nanoparticles.
  • Next-generation sequencing of 16S ribosomal DNA for microbial community analysis.
  • Applying thermodynamic, electrostatic, and chemical bonding principles to model HAp-bacteria interactions.

Main Results:

  • HAp nanoparticles significantly reduced Gram-negative bacteria while increasing Gram-positive bacteria.
  • Bacterial motility (flagella) and cell surface properties (lipoteichoic acid, lipopolysaccharide) influence HAp binding.
  • HAp preferentially bound less pathogenic bacteria and agriculturally beneficial taxa.

Conclusions:

  • HAp nanoparticles demonstrate selective antibacterial activity, altering microbiome composition.
  • The findings suggest potential environmental and biomedical applications for HAp in microbial control.
  • HAp's selective sequestration of Gram-negative bacteria offers a novel approach to microbiome management.