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Updated: Aug 29, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Complete mitochondrial genome sequence of Pulveroboletus ravenelii (Boletales, Basidiomycota)
Sung-Eun Cho1, Young-Nam Kwag1, Sang-Kuk Han1
1Forest Biodiversity Division, Korea National Arboretum, Pocheon, South Korea.
Abstract:
Pulveroboletus ravenelii, an ectomycorrhizal fungus, has long been used in traditional Chinese medicine and mushroom dyes; however, its mitogenome and phylogenetic relationship with other taxa remain unexplored. Here, we sequenced the mitochondrial genome of P. ravenelii using next-generation sequencing and found that its mitogenome, a circular DNA molecule of 43,528 bp, comprised 15 protein-coding genes, 15 transfer RNA genes, and two ribosomal RNA genes. The mitogenome had a base composition of A (37.52%), C (11.14%), G (12.21%), and T (39.13%) and a GC content of 23.35%. A phylogenetic tree was constructed to determine the phylogenetic relationship of this species with other taxa based on the mitochondrial genome sequence. This study revealed the phylogenetic positions of P. ravenelii and its related genera for the first time.
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