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Related Concept Videos

Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Updated: Aug 29, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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On-DNA Suzuki-Miyaura Cross-Coupling.

Yun Ding1

  • 1Encoded Library Technologies/NCE Molecular Discovery, R&D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, MA, USA. yun.x.ding@gsk.com.

Methods in Molecular Biology (Clifton, N.J.)
|September 9, 2022
PubMed
Summary

This study introduces an efficient Suzuki-Miyaura cross-coupling method for on-DNA halide substrates. The reaction utilizes palladium tetrakis(triphenylphosphine) as a catalyst for broad applications in medicinal chemistry.

Keywords:
DNA-encoded library (DEL)PalladiumSuzuki-Miyaura cross-coupling

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Area of Science:

  • Organic Chemistry
  • Medicinal Chemistry
  • Biochemistry

Background:

  • Suzuki-Miyaura cross-coupling is a cornerstone reaction in modern organic synthesis.
  • Its application in modifying DNA directly presents unique challenges and opportunities.

Purpose of the Study:

  • To develop an efficient method for Suzuki-Miyaura cross-coupling directly on DNA-bound halide substrates.
  • To expand the toolkit for DNA functionalization in medicinal chemistry and beyond.

Main Methods:

  • Utilized palladium tetrakis(triphenylphosphine) [Pd(PPh3)4] as the coupling catalyst.
  • Employed on-DNA halide substrates for the cross-coupling reaction.

Main Results:

  • Achieved efficient Suzuki-Miyaura cross-coupling on DNA halide substrates.
  • Demonstrated the utility of Pd(PPh3)4 for this specific application.

Conclusions:

  • The described method offers a novel and efficient route for the direct functionalization of DNA.
  • This advancement holds significant potential for applications in drug discovery and chemical biology.