In-vitro cytotoxicity of nickel oxide nanoparticles against L-6 cell-lines: MMP, MTT and ROS studies

Area of Science:

• Nanotechnology
• Materials Science
• Biotechnology

Background:

• Nickel oxide nanoparticles (NiO NPs) are explored for various applications.
• Green synthesis offers a sustainable approach to nanoparticle production.
• Plant extracts can act as capping and stabilizing agents in nanoparticle synthesis.

Purpose of the Study:

• To synthesize NiO NPs using four different plant extracts: Rhododendron arboretum, Tinospora cordifolia, Corylus jacquemontii, and Nardostachys jatamansi.
• To characterize the synthesized NiO NPs for their physical and chemical properties.
• To evaluate the antiproliferative activity of the synthesized NiO NPs against rat skeletal myoblast (L-6) cell lines.

Main Methods:

• Co-precipitation method for NiO NP synthesis using plant extracts.
• Characterization using techniques to determine morphology, crystallinity, and structure.
• In vitro cytotoxicity assays on L-6 cell lines to assess antiproliferative effects.

Main Results:

• Successful synthesis of NiO NPs confirmed by morphological studies.
• Structural and compositional analysis indicated high purity and good crystallinity.
• Tinospora cordifolia derived NiO NPs demonstrated significant antiproliferative activity with an IC50 of 1.671 mg/mL, outperforming other plant extracts.

Conclusions:

• Plant-mediated synthesis of NiO NPs is an effective and eco-friendly approach.
• Tinospora cordifolia derived NiO NPs show potent antiproliferative properties against L-6 cells.
• Synthesized NiO NPs hold potential as antiproliferative agents for further investigation.