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Ara H1 peanut allergen detection using a labelled electrochemical aptasensor based on GO-COOH@bimetallic composite
Gheorghe Melinte1, Oana Hosu2, Cecilia Cristea2
1Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy; Department of Analytical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 4 Pasteur Street, 400349 Cluj-Napoca, Romania.
Abstract:
Allergies are defined as a hypersensitivity reaction, immunologically mediated, as a result to an external stimulus. Peanuts induced allergies are considered one of the most severe, life-threatening food sensitivities since they trigger the highest frequency of severe and fatal reactions, even in trace amounts. Therefore, it is imperative to develop fast, accurate and easy-to-use analytical methods to determine Ara h1, is a seed storage protein from Arachis hypogea and the main peanut derived allergen. In this work, two strategies were applied to develop an electrochemical aptasensor based on GO-COOH and metallic nanoparticles immobilised on screen-printed carbon electrodes (SPCEs). The analytical performances of the aptasensor showed a linear range of 5-150 nM, and a limit of detection of 1.66 nM. The method was applied in peanut-free food samples with very good recoveries proving to be a promising tool for peanut allergy prevention.
