Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental microbial bioprospecting enabled by a Raman fingerprinting and functional sorting on a microfluidic static droplet array.

Analytica chimica acta·2026
Same author

A microfluidic biomimetic system from the blood-brain barrier to activated microglia towards anti-Mesial Temporal Lobe Epilepsy drug screening.

Analytical and bioanalytical chemistry·2025
Same author

Cortical compensation mechanism for swallowing recovery in patients with medullary infarction-induced dysphagia.

Frontiers in neurology·2024
Same author

Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae.

Bioactive materials·2023
Same author

RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication.

Frontiers in microbiology·2022
Same author

Instrumentation-Compact Digital Microfluidic Reaction Interface-Extended Loop-Mediated Isothermal Amplification for Sample-to-Answer Testing of <i>Vibrio parahaemolyticus</i>.

Analytical chemistry·2021

Related Experiment Video

Updated: Aug 29, 2025

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.4K

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae.

Guoxia Zheng1, Furong Gu1, Yutong Cui1

  • 1Environmental and Chemical Engineering Institute, Dalian University, Dalian, 116622, China; Dalian Key Laboratory of Oligosaccharide Recombination and Recombinant Protein Modification,Dalian, 116622, China.

Analytica Chimica Acta
|September 11, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic droplet platform for high-throughput screening of microalgae, accelerating the identification of efficient strains for renewable oil production. The system enables real-time monitoring of individual cells, revealing lipid accumulation and proliferation patterns.

Keywords:
BiofuelsMicroalgaeMicrofluidic dropletScreeningSingle-cell-analysis

More Related Videos

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.8K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.4K

Related Experiment Videos

Last Updated: Aug 29, 2025

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

10.4K
Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

1.8K
Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
08:20

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Published on: February 22, 2016

10.4K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Renewable Energy

Background:

  • Microalgae are promising feedstocks for renewable oil but require efficient screening methods.
  • Current methods for selecting microalgae species and optimizing culture conditions are laborious and slow.

Purpose of the Study:

  • To develop a high-throughput microfluidic platform for rapid screening and analysis of individual microalgae.
  • To enable real-time monitoring of lipid accumulation and cell proliferation at the single-cell level.

Main Methods:

  • A microfluidic droplet-based system with a resolution of 2 x 10^3 was developed.
  • The platform integrates novel fluid-blocking-based droplet generation and trapping for operational simplicity, rapidity, and stability.
  • The system was used to screen three unicellular algae species: Isochrysis zhanjiangensis, Platymonas subcordiformis, and Platymonas helgolandica var. tsingtaoensis.

Main Results:

  • The platform successfully enabled in situ bioassays of lipid accumulation and cell proliferation at the single-cell level for interspecies comparison.
  • Lipid production inhomogeneity was observed among cells within the same species and batch.
  • Nitrogen stress was identified as a condition inducing a positive-skewed frequency distribution of lipid content, even in inhomogeneous individual cells.

Conclusions:

  • The developed microfluidic platform significantly accelerates microalgae screening and analysis.
  • The system provides valuable insights into single-cell lipid production dynamics and responses to environmental conditions.
  • This technology holds great potential for single-cell isolation and screening, advancing renewable oil production from microalgae.