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Plastic-adherent progenitor cells in human bone marrow
Abstract:
Human bone marrow contains plastic-adherent hemopoietic progenitor cells whose plating efficiency is increased by brief (2 h) exposure to methylprednisolone (MP). When subsequently covered with methylcellulose medium, they form colonies of monoblastoid cells. Colony size, but not number, and mature cell production are increased by erythropoietin (epo) and granulocyte-macrophage colony-stimulating factor (GM-CSF). However, colonies do not grow under serum-free conditions. The resistance of plastic-adherent progenitors to treatment with 5-fluorouracil (5FU), their growth pattern, and their capacity to produce granulocytic and erythroid colonies on replating, suggest that they may be similar to the primitive, 5FU-resistant, plastic-adherent progenitor cells (HPP-CFC) in murine marrow.
Insights
Methylprednisolone enhances human bone marrow progenitor cell growth. These cells, similar to primitive murine cells, form colonies but require serum for growth.
Area of Science:
- Hematology
- Stem Cell Biology
- Cellular Biology
Background:
- Human bone marrow harbors plastic-adherent hematopoietic progenitor cells.
- These progenitors exhibit specific responses to certain growth factors and chemical treatments.
Purpose of the Study:
- To characterize human bone marrow plastic-adherent progenitor cells.
- To investigate the effects of methylprednisolone, erythropoietin, and GM-CSF on these cells.
- To compare human progenitors with murine primitive progenitor cells.
Main Methods:
- Exposure of human bone marrow cells to methylprednisolone.
- Culturing cells in methylcellulose medium with specific growth factors.
- Assessment of colony formation, size, and mature cell production.
- Evaluation of progenitor resistance to 5-fluorouracil (5FU).
Main Results:
- Methylprednisolone (MP) treatment increased plating efficiency of plastic-adherent progenitors.
- Erythropoietin (epo) and granulocyte-macrophage colony-stimulating factor (GM-CSF) increased colony size and mature cell production, but not colony number.
- Colony growth was dependent on serum presence; serum-free conditions inhibited growth.
- Progenitors demonstrated resistance to 5-fluorouracil (5FU).
Conclusions:
- Human bone marrow plastic-adherent progenitors share characteristics with primitive, 5FU-resistant, murine progenitor cells (HPP-CFC).
- These findings contribute to understanding early hematopoiesis and progenitor cell behavior.