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Related Experiment Videos

Plastic-adherent progenitor cells in human bone marrow.

M Y Gordon, G P Riley, M F Greaves

    Experimental Hematology
    |August 1, 1987
    PubMed
    Summary

    Methylprednisolone enhances human bone marrow progenitor cell growth. These cells, similar to primitive murine cells, form colonies but require serum for growth.

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    Area of Science:

    • Hematology
    • Stem Cell Biology
    • Cellular Biology

    Background:

    • Human bone marrow harbors plastic-adherent hematopoietic progenitor cells.
    • These progenitors exhibit specific responses to certain growth factors and chemical treatments.

    Purpose of the Study:

    • To characterize human bone marrow plastic-adherent progenitor cells.
    • To investigate the effects of methylprednisolone, erythropoietin, and GM-CSF on these cells.
    • To compare human progenitors with murine primitive progenitor cells.

    Main Methods:

    • Exposure of human bone marrow cells to methylprednisolone.
    • Culturing cells in methylcellulose medium with specific growth factors.
    • Assessment of colony formation, size, and mature cell production.
    • Evaluation of progenitor resistance to 5-fluorouracil (5FU).

    Main Results:

    • Methylprednisolone (MP) treatment increased plating efficiency of plastic-adherent progenitors.
    • Erythropoietin (epo) and granulocyte-macrophage colony-stimulating factor (GM-CSF) increased colony size and mature cell production, but not colony number.
    • Colony growth was dependent on serum presence; serum-free conditions inhibited growth.
    • Progenitors demonstrated resistance to 5-fluorouracil (5FU).

    Conclusions:

    • Human bone marrow plastic-adherent progenitors share characteristics with primitive, 5FU-resistant, murine progenitor cells (HPP-CFC).
    • These findings contribute to understanding early hematopoiesis and progenitor cell behavior.

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