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Ascaris suum: human lymphocyte mitogenic factor content.

S Sasagawa, K Suzuki, T Fujikura

    Experimental Parasitology
    |August 1, 1987
    PubMed
    Summary

    Ascaris suum extract contains a substance that stimulates human lymphocytes, promoting cell division, particularly in T lymphocytes. This mitogenic factor remains active even after heat treatment and can be separated from allergenic components.

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    Area of Science:

    • Immunology
    • Parasitology
    • Biochemistry

    Background:

    • Ascaris suum is a common parasitic roundworm.
    • Parasitic infections can modulate host immune responses.

    Purpose of the Study:

    • To investigate the immunomodulatory properties of Ascaris suum extract.
    • To identify and characterize mitogenic factors in the extract.

    Main Methods:

    • Human lymphocytes were stimulated with Ascaris suum extract.
    • Mitogenic activity was assessed by [3H]thymidine incorporation.
    • Heat stability and fractionation using Sephacryl S-200 chromatography were performed.
    • Allergenic activity was tested using the passive cutaneous anaphylaxis test.

    Main Results:

    • Ascaris suum extract demonstrated significant mitogenic activity on human lymphocytes, comparable to pokeweed mitogen.
    • T lymphocytes showed a more pronounced response to the extract.
    • The mitogenic activity was relatively heat-stable.
    • Fractionation separated the mitogenic activity from allergenic activity within the same protein peak.

    Conclusions:

    • Ascaris suum extract contains a heat-stable mitogenic substance that stimulates human lymphocytes, primarily T cells.
    • The extract possesses both mitogenic and allergenic properties, which can be chromatographically separated.
    • These findings contribute to understanding the complex immune interactions between Ascaris suum and its host.

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