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Updated: Aug 29, 2025

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation

Published on: November 17, 2014

Microbiological Testing of Split-Cornea Transplantation.

Anne Tillmann¹, Katja Rosenbaum¹, Gerd Geerling¹

¹Department of Ophthalmology, University of Düsseldorf, Düsseldorf, Germany.

Current Eye Research

|September 12, 2022

Summary

This summary is machine-generated.

Splitting donor corneas for transplantation can help address donor shortages. Microbiological testing confirmed that using anterior lamellae after storage is a safe procedure for patients.

Keywords:

DALK DMEK Split-cornea transplantation eye bank

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Last Updated: Aug 29, 2025

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Area of Science:

Ophthalmology
Transplantation Medicine
Microbiology

Background:

Donor cornea scarcity poses a significant challenge in eye banking.
Current regulations restrict each donor cornea to a single recipient, limiting tissue availability.
Corneal transplantation, particularly Descemet Membrane Endothelial Keratoplasty (DMEK), relies heavily on donor tissue.

Purpose of the Study:

To evaluate the microbiological safety of using anterior donor lamellae for split-cornea transplantation.
To assess the feasibility of reducing donor tissue demand by utilizing both anterior and posterior lamellae from a single donor cornea.
To challenge existing eye bank regulations that limit cornea utilization.

Main Methods:

Anterior lamellae, not used in sterile DMEK procedures, were stored for one week.
Microbiological cultures were performed on stored anterior lamellae on day 7 post-storage.
108 consecutive anterior lamellae underwent microbiological assessment between October 2020 and December 2021.

Main Results:

No microbial contamination was detected in any of the 108 anterior lamellae tested.
The microbiological controls performed at the University of Duesseldorf showed zero contamination.
This indicates a high safety profile for stored anterior lamellae.

Conclusions:

Split-cornea transplantation, utilizing anterior lamellae after a storage period, is microbiologically safe.
This approach offers a viable strategy to mitigate donor cornea shortages.
The findings support a re-evaluation of current eye bank regulations regarding cornea utilization.