Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.2K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASO Visual Abstract: Longitudinal Symptom Recovery After Pancreatectomy-A Prospective Patient-Reported Outcomes Study using MDASI.

Annals of surgical oncology·2026
Same author

Longitudinal Symptom Recovery After Pancreatectomy: A Prospective Patient-Reported Outcomes Study using MDASI.

Annals of surgical oncology·2026
Same author

An innovative technology boosts image quality for protein structures.

Nature·2026
Same author

'Virtual cells' aim to turn raw data into predictive models of biology.

Nature·2026
Same author

A 3D-Printed Scaffolded Hydrogel Microneedle Array Biosensor for Real-Time, Continuous Monitoring.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Continuous monitoring of blood-interstitial fluid intercompartmental molecular kinetics in freely moving animals.

Science advances·2026
Same journal

Chlorinated VSLSs Surpass HCFCs in CFC-11-Equivalent Emissions for Ozone Layer Depletion in China.

Nature communications·2026
Same journal

Author Correction: Charge transfer in triphenylamine-tetrazine covalent organic frameworks for solar-driven hydrogen peroxide production.

Nature communications·2026
Same journal

Vegetation browning patterns under compound soil and atmospheric dryness in northern permafrost ecosystems.

Nature communications·2026
Same journal

Voltage imaging of CA1 pyramidal cells and SST+ interneurons reveals stability and plasticity mechanisms of spatial firing.

Nature communications·2026
Same journal

Radical-omics reveals the hydrogen-abstraction pathway of isoprene oxidation.

Nature communications·2026
Same journal

Toughening elastomer via sequentially activated multi-pathway energy dissipation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Aug 29, 2025

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

12.1K

Improved immunoassay sensitivity and specificity using single-molecule colocalization.

Amani A Hariri1, Sharon S Newman2,3, Steven Tan4

  • 1Department of Radiology, Stanford University, Stanford, CA, 94305, USA.

Nature Communications
|September 13, 2022
PubMed
Summary
This summary is machine-generated.

A new Single-Molecule Colocalization Assay (SiMCA) improves molecular detection by reducing background noise. This novel immunoassay method enhances sensitivity and accuracy in complex biological samples.

More Related Videos

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

374
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.6K

Related Experiment Videos

Last Updated: Aug 29, 2025

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

12.1K
High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research
09:12

High-Throughput Automated Multiplex Immunofluorescence Assays for Translational Research

Published on: June 10, 2025

374
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.6K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Enzyme-linked immunosorbent assays (ELISAs) are widely used but challenged by non-specific background noise.
  • Distinguishing true molecular signals from background is crucial for accurate detection.
  • Non-specific binding can significantly impair immunoassay performance.

Purpose of the Study:

  • To develop a novel assay that overcomes the limitations of traditional immunoassays.
  • To reduce the impact of non-specific background noise in molecular detection.
  • To improve the sensitivity and reliability of protein quantification.

Main Methods:

  • Development of a Single-Molecule Colocalization Assay (SiMCA).
  • Utilizing total internal reflection fluorescence microscopy for signal detection.
  • Quantifying target proteins via colocalization of orthogonally labeled antibodies.

Main Results:

  • SiMCA effectively eliminates background from non-specific antibody binding.
  • Achieved a three-fold lower limit of detection for TNF-α compared to single-color assays.
  • Demonstrated consistent performance in complex specimens like serum and blood.

Conclusions:

  • SiMCA offers significant diagnostic gains by mitigating non-specific background effects.
  • The assay provides a more accurate and sensitive method for molecular detection.
  • This approach enhances the reliability of immunoassays in complex biological matrices.