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One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
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Published on: January 26, 2024

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Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system

Abderhman Abuhashem1, Anna-Katerina Hadjantonakis2

  • 1Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program, New York, NY 10065, USA; Biochemistry Cell and Molecular Biology Program, Weill Cornell Graduate School of Medical Sciences, Cornell University, New York, NY 10065, USA.

STAR Protocols
|September 13, 2022
PubMed

Keywords:
CRISPRCell cultureDevelopmental biologyGeneticsMicroscopyModel OrganismsMolecular BiologyStem Cells

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