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In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
Published on: November 17, 2018
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A Nanozyme-Immobilized Hydrogel with Endogenous ROS-Scavenging and Oxygen Generation Abilities for Significantly
Zuhao Li1, Yue Zhao2,3, Hanwei Huang4
1Orthopaedic Medical Center, The Second Hospital of Jilin University, Orthopaedic Research Institute of Jilin Province, No. 218 Ziqiang Street, Changchun, 130041, China.
Advanced Healthcare Materials
|September 13, 2022
Summary
Engineered hydrogels combat diabetic wound healing challenges by neutralizing reactive oxygen species (ROS) and producing oxygen. This promotes cell survival and accelerates wound closure, offering a novel therapeutic approach.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Diabetic non-healing wounds present significant morbidity and mortality.
- Existing hydrogels are suboptimal due to high reactive oxygen species (ROS) and chronic hypoxia in diabetic wound environments.
- A novel approach is needed to ameliorate the hostile diabetic wound microenvironment.
Purpose of the Study:
- To develop a metabolism-inspired hydrogel capable of addressing ROS accumulation and hypoxia in diabetic wounds.
- To engineer nanozyme-reinforced hydrogels with ROS-scavenging and oxygen-producing capabilities.
- To evaluate the efficacy of these hydrogels in promoting diabetic wound healing.
Main Methods:
- Fabrication of hydrogels using modified hyaluronic acid polymers and a metal-organic framework-derived nanozyme (ε-polylysine coated mesoporous manganese cobalt oxide).
- Assessment of the hydrogels' ability to capture ROS and produce oxygen.
- In vitro evaluation of hydrogel effects on skin cells (keratinocytes, fibroblasts, vascular endothelial cells).
- In vivo assessment of hydrogel dressings on diabetic wound healing, including inflammatory response, cell proliferation, re-epithelialization, collagen deposition, and neovascularization.
Main Results:
- The nanozyme-reinforced hydrogels effectively captured ROS and produced oxygen, mitigating oxidative stress and hypoxia.
- Hydrogels protected skin cells from ROS and hypoxia-induced damage and proliferation inhibition.
- Treatment with hydrogels promoted a shift in macrophage polarization from M1 to M2 phenotype.
- Significantly accelerated wound healing was observed, characterized by reduced inflammation, enhanced proliferation, re-epithelialization, collagen deposition, and neovascularization.
Conclusions:
- Nanozyme-reinforced hydrogels serve as effective ROS-driven oxygenerators for ameliorating the diabetic wound microenvironment.
- This strategy enhances diabetic wound healing by protecting cells, modulating immune response, and promoting tissue regeneration.
- The developed hydrogel dressing represents a promising therapeutic for improving outcomes in diabetic wound patients.
