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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Introduction: Cryo-EM in Biology and Materials Research

Sriram Subramaniam1, Dganit Danino2

  • 1The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.

Chemical Reviews
|September 14, 2022
PubMed
Abstract

No abstract available in PubMed .

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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