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Confined Nanopipet as a Versatile Tool for Precise Single Cell Manipulation.

Ru-Jia Yu^1,2,3, Yong-Xu Hu³, Ke-Le Chen¹

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, People's Republic of China.

Analytical Chemistry

|September 14, 2022

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This study introduces a nanopipet method for precise single-cell manipulation. This technique allows for femtoliter-scale cytoplasm extraction and analysis, aiding in understanding cellular heterogeneity.

Area of Science:

Cell Biology
Analytical Chemistry
Biotechnology

Background:

Precise single-cell manipulation is vital for understanding cellular mechanisms and heterogeneity.
Advanced analytical methods like single-cell omics can reveal hidden cellular diversity.
Current methods may lack the precision for ultrasmall volume manipulation.

Purpose of the Study:

To develop a versatile nanopipet-based method for precise single-cell manipulation.
To enable ultrasmall volume liquid handling for single-cell analysis.
To demonstrate applications in cytoplasm extraction and component injection.

Main Methods:

Utilized a nanopipet system for ultrasmall liquid volume manipulation.
Extracted femtoliter volumes of cytoplasm from single living cells.

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Analyzed extracted cytoplasm using time-of-flight secondary ion mass spectrometry (TOF-SIMS).

Injected multiple exogenous components into single living cells.

Main Results:

Demonstrated precise manipulation of single cells using the nanopipet method.
Successfully extracted and analyzed femtoliter volumes of cytoplasm.
Showcased the capability for simultaneous injection of multiple exogenous components into cells.
Enabled delicate multi-imaging in single living cells.

Conclusions:

The nanopipet method offers a versatile tool for precise single-cell manipulation.
This technique facilitates detailed analysis of cellular components and heterogeneity.
It provides a delicate platform for advanced cellular studies, including multi-imaging and component delivery.