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Updated: Aug 28, 2025
06:44
Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups
Published on: April 6, 2017
9.8K
Water-soluble nickel (II) Schiff base complexes: Synthesis, structural characterization, DNA binding affinity, DNA
Fatemeh Sadat Dehghani1, Razieh Kalantari1, Banafsheh Rastegari2
1Department of Chemistry, School of Sciences, Shiraz University, Shiraz, Iran.
Nucleosides, Nucleotides & Nucleic Acids
|September 15, 2022
Summary
This study synthesized two nickel (II) Schiff base complexes and investigated their DNA binding. Complex 1 showed stronger DNA binding and comparable toxicity to cisplatin, suggesting partial intercalation as the primary binding mode.
Area of Science:
- Coordination Chemistry
- Bioinorganic Chemistry
- Materials Science
Background:
- Schiff base complexes are investigated for their potential biological applications, including DNA interaction.
- Understanding the mechanism of DNA binding is crucial for developing novel therapeutic agents.
- Nickel(II) complexes offer unique electronic and structural properties for bioinorganic studies.
Purpose of the Study:
- To synthesize and characterize novel water-soluble nickel (II) Schiff base complexes.
- To investigate the DNA binding interaction of these complexes using various biophysical techniques.
- To compare the DNA binding affinity and mechanism of two distinct nickel (II) complexes.
Main Methods:
- Synthesis and characterization of nickel (II) Schiff base complexes (Complex 1 and Complex 2).
- UV-vis absorption and fluorescence spectroscopy for DNA binding studies.
- Cyclic voltammetry, viscometric measurements, and molecular docking for mechanistic insights.
- Cell toxicity assays (Jurkat, MCF-7) and microscopy for biological evaluation.
Main Results:
- Complex 1 exhibited stronger binding affinity to fish sperm DNA (FS-DNA) than Complex 2.
- Thermodynamic parameters indicated hydrophobic interactions for Complex 1 and electrostatic interactions for Complex 2 with DNA.
- Complex 1 demonstrated comparable cytotoxicity to cisplatin against cancer cell lines, with evidence of DNA cleavage.
Conclusions:
- Both complexes bind to DNA via partial intercalation, with Complex 1 showing superior binding.
- The findings highlight the potential of nickel (II) Schiff base complexes as DNA-interacting agents.
- Complex 1's comparable toxicity to cisplatin warrants further investigation for anticancer drug development.
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