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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Updated: Aug 28, 2025

Generation of Fluorescent Protein Fusions in Candida Species
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Using FPbase: The Fluorescent Protein Database.

Talley J Lambert1

  • 1Department of Cell Biology and Department of Systems Biology, Harvard Medical School, Boston, MA, USA. talley@hms.harvard.edu.

Methods in Molecular Biology (Clifton, N.J.)
|September 15, 2022
PubMed
Summary
This summary is machine-generated.

FPbase provides a comprehensive database of fluorescent proteins and associated tools for researchers. It aids in searching protein characteristics and utilizing experimental tools like the spectra viewer and FRET calculator.

Keywords:
DatabaseFluorescent proteinsSpectraVisualization

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Fluorescent proteins (FPs) are crucial tools in biological research for imaging and tracking.
  • A centralized, searchable database with integrated tools is needed to efficiently utilize FP characteristics.

Purpose of the Study:

  • To provide a comprehensive reference for the FPbase database and its associated online tools.
  • To guide users in searching the database and employing tools for experimental design.

Main Methods:

  • Database curation of fluorescent protein properties.
  • Development of online tools: spectra viewer, custom microscope pages, FRET calculator.
  • Inclusion of data evaluation guidelines.

Main Results:

  • FPbase offers a searchable repository of fluorescent proteins and their spectral and functional characteristics.
  • Integrated tools facilitate data visualization (spectra viewer) and experimental planning (FRET calculator, custom microscope configurations).

Conclusions:

  • FPbase serves as a valuable resource for researchers working with fluorescent proteins.
  • The database and tools streamline the selection and application of fluorescent probes in diverse biological experiments.