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Color-Switchable Nanosilicon Fluorescent Probes.

Huai Chen¹, Jiang Xu², Yaping Wang¹

¹MOE Laboratory of Bioinorganic and Synthetic Chemistry, Lehn Institute of Functional Materials, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China.

|September 15, 2022

Summary

This summary is machine-generated.

Researchers developed novel color-switchable probes (CSPs) using silicon nanocrystals (SiNCs) for live cell imaging. These probes detect amino acids in real-time, offering a new tool for cellular analysis.

Keywords:

bioimaging color-switchable probe nanocrystal photoluminescence silicon surface chemistry

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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Area of Science:

Nanotechnology
Cell Biology
Biochemistry

Background:

Fluorescent probes are essential for cell imaging and quantification.
Color-switchable probes (CSPs) offer advantages like reduced washing steps and internal controls for concentration.
Existing CSPs do not cover all critical cellular targets.

Purpose of the Study:

To develop a novel CSP for in situ cell imaging.
To utilize aldehyde-functionalized silicon nanocrystals (SiNCs) for this purpose.
To enable visualization and quantification of amino acids in live cells.

Main Methods:

Synthesized aldehyde-functionalized silicon nanocrystals (SiNCs).
Investigated the photoluminescence switching behavior of SiNCs upon interaction with amino acids.
Assessed SiNC performance in live cells at 37 °C and in cell-free extracts at varying temperatures.
Determined SiNC localization within cells (cytoplasm vs. nucleus).
Correlated SiNC fluorescence intensity with amino acid concentration.

Main Results:

Developed SiNCs that function as CSPs, switching fluorescence from red to blue upon amino acid interaction.
SiNCs demonstrated superior performance in live cells at 37 °C compared to cell-free extracts.
SiNCs localized specifically in the cytoplasm, excluding the nucleus.
A linear correlation was observed between SiNC fluorescence intensity and amino acid concentration.

Conclusions:

Aldehyde-functionalized SiNCs represent a pioneering CSP for live cell imaging.
These SiNCs offer rapid and quantitative detection of amino acids in cellular environments.
The developed probes show promise for visualizing amino acid distribution and quantifying related cellular processes.