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Fibronectins Connect Cells with ECM01:25

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Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
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Updated: Aug 28, 2025

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
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Bioactive Fibronectin-III10-DNA Origami Nanofibers Promote Cell Adhesion and Spreading.

Alex Buchberger1,2, Kyle Riker3, Julio Bernal-Chanchavac1,2

  • 1School of Molecular Sciences, Arizona State University, Tempe, Arizona 85281, United States.

ACS Applied Bio Materials
|September 15, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method to integrate bioactive fibronectin proteins with DNA nanostructures. This creates advanced protein-DNA nanofibers with enhanced bioactivity for biomaterial applications.

Keywords:
DNA nanotechnologybiomaterialsbionanotechnologycoiled-coilsfibronectinself-assemblysupramolecular chemistry

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Biology

Background:

  • Integrating proteins with DNA nanostructures offers potential for advanced materials.
  • Fibronectin domain proteins are crucial for cell adhesion and tissue engineering.

Purpose of the Study:

  • To develop a method for incorporating bioactive fibronectin proteins into DNA nanostructures.
  • To create self-assembled, one-dimensional protein-DNA nanofibers.
  • To evaluate the bioactivity of these novel nanofibers.

Main Methods:

  • Utilized two orthogonal coiled-coil peptides for protein-DNA conjugation.
  • Attached peptides to DNA origami cuboids via DNA handles.
  • Assembled nanostructures into 1D arrays using fibronectin domain linkers.
  • Characterized array formation using atomic force and electron microscopy.

Main Results:

  • Successfully created self-assembled, one-dimensional protein-DNA nanofibers.
  • Validated array formation through distinct self-assembly protocols.
  • Demonstrated enhanced fibroblast adhesion and spreading on protein-DNA nanofiber-coated surfaces.
  • Observed superior bioactivity compared to monomeric fibronectin protein.

Conclusions:

  • The developed method enables the creation of bioactive protein-DNA nanofibers.
  • These nanofibers show promise as advanced biomaterial substrates.
  • The integration strategy enhances protein bioactivity for diverse applications.