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Quantifying active and resistive stresses in adherent cells.
Hélène Delanoë-Ayari1, Alice Nicolas2
1Université de Lyon, Université Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, F-69622, VILLEURBANNE, France.
Physical Review. E
|September 16, 2022
Summary
Understanding cell migration requires measuring intracellular stresses. New 3D modeling reveals current methods calculate different stress components, but combining them could isolate active cell stress.
Area of Science:
- Biophysics
- Cell Biology
- Mechanobiology
Background:
- Cell migration is fundamental to biological processes.
- Accurate measurement of intracellular stresses is crucial for modeling cell movement.
- Existing methods for stress calculation operate under thin plate approximations.
Purpose of the Study:
- To investigate and differentiate the quantities calculated by existing intracellular stress measurement techniques.
- To determine if combining different methods can isolate specific stress components.
Main Methods:
- Three-dimensional Finite Element Method (3D FEM) modeling was employed.
- Simulations were performed to compare monolayer stress microscopy (MSM), Bayesian inference stress microscopy (BISM), and intracellular stress microscopy (ISM).
Main Results:
- 3D FEM modeling demonstrated that MSM and BISM calculate the sum of active and resistive stresses.
- ISM was shown to calculate only the resistive stress component.
- The study revealed that current techniques do not measure the same quantities as previously assumed.
Conclusions:
- Different established methods yield distinct intracellular stress measurements.
- Combining MSM/BISM with ISM offers a potential pathway to isolate and quantify active cellular stress.
- This differentiation is key for advancing biophysical models of cell migration.
