Related Experiment Video

Updated: Aug 28, 2025

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.7K

Naringin Inhibits the Proliferation, Migration, Invasion and Epithelial-to-Mesenchymal Transition of Gastric Cancer

Lin Zhu, Jing Shi, Mingchao Mu

    Alternative Therapies in Health and Medicine
    |September 16, 2022
    PubMed
    Abstract

    Insights

    Naringin effectively inhibits gastric cancer growth by halting cell-cycle progression, inducing apoptosis, and suppressing epithelial mesenchymal transition (EMT) via the PI3K-AKT/Zeb1 pathway.

    Area of Science:

    • Oncology
    • Pharmacology
    • Traditional Chinese Medicine

    Background:

    • Gastric cancer is a prevalent digestive system malignancy.
    • Current treatments include radiotherapy, chemotherapy, and surgery.
    • Exploring novel therapeutic strategies, including Traditional Chinese Medicine (TCM), is crucial.

    Purpose of the Study:

    • To investigate the effects of naringin on gastric cancer cell proliferation, migration, invasion, and apoptosis.
    • To elucidate the underlying molecular mechanisms of naringin's action in gastric cancer.

    Main Methods:

    • Cell viability assessed by MTT assay.
    • Cloning, migration, and invasion evaluated using colony formation, scratch, and Transwell assays.
    • Cell cycle and apoptosis analyzed via flow cytometry; protein expression by Western blot and IHC.
    • In vivo efficacy tested in a nude mouse model.

    Main Results:

    • Naringin treatment arrested the cell cycle in the G0/G1 phase and increased apoptosis.
    • Tumor growth was significantly inhibited in nude mice.
    • Gastric cancer cell invasion and migration were markedly reduced.
    • Expression of Vimentin, Zeb1, and P-AKT decreased, while E-cadherin increased.

    Conclusions:

    • Naringin inhibits gastric cancer development by blocking the cell cycle and inducing apoptosis.
    • Naringin suppresses epithelial mesenchymal transition (EMT) by inhibiting the PI3K-AKT/Zeb1 pathway.
    • Naringin demonstrates potential as a therapeutic agent for gastric cancer.

    More Related Videos

    Preparation of Naringenin Solution for In Vivo Application08:18

    Preparation of Naringenin Solution for In Vivo Application

    Published on: August 10, 2021

    3.4K
    Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma13:18

    Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

    Published on: March 3, 2023

    1.3K

    Related Concept Videos

    mTOR Signaling and Cancer Progression03:03

    mTOR Signaling and Cancer Progression

    The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
    The mTOR pathway or the...
    3.9K
    PI3K/mTOR/AKT Signaling Pathway01:22

    PI3K/mTOR/AKT Signaling Pathway

    The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
    3.9K
    Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists01:28

    Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

    Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy.  SP binds and activates...
    250
    Inhibition of Cdk Activity02:34

    Inhibition of Cdk Activity

    The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
    4.9K
    Interactions Between Signaling Pathways01:19

    Interactions Between Signaling Pathways

    Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
    Convergence and divergence, and cross-talk between signaling pathways
    Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
    6.4K
    NF-κB-dependent Signaling Pathway02:26

    NF-κB-dependent Signaling Pathway

    The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
    NF-κB-dependent Signaling Mechanism
    The...
    7.8K