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Single-cell scanning photoelectrochemical microscopy using micro-optical-ring electrodes.

Nikita Thomas1, Vikram Singh1, Nafisa Ahmed1

  • 1Laboratory for Bioanalytics and Electrochemical Sensing, Department of Chemistry, University of Manitoba, Winnipeg, R3T 2N2, Canada.

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|September 17, 2022
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Summary
This summary is machine-generated.

Researchers developed a cost-effective method for fabricating micro-optical-ring electrodes (MOREs). These MOREs enable simultaneous optical and electrochemical detection, advancing applications in scanning photoelectrochemical microscopy (SPECM) and live cell imaging.

Keywords:
Local illuminationMicro-optical-ring electrodeMicroelectrodeScanning photoelectrochemical microscopySingle cellSpectroelectrochemistry

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biophysics

Background:

  • Microelectrodes are vital analytical tools with diverse applications.
  • Micro-optical-ring electrodes (MOREs) combine electrochemical sensing with optical signal transmission.
  • Advancements in probe design are crucial for high-resolution imaging and analysis.

Purpose of the Study:

  • To present a user-friendly and cost-effective fabrication method for MOREs.
  • To enable scanning photoelectrochemical microscopy (SPECM) applications using MOREs.
  • To demonstrate the utility of MOREs for spectro-electrochemical detection and live cell imaging.

Main Methods:

  • Fabrication of micro-scale ring-electrodes with integrated optical fiber cores.
  • Characterization using electrochemistry, numerical modeling, and scanning electron microscopy (SEM).
  • Integration into scanning probe microscopy for spectro-electrochemical measurements.

Main Results:

  • Reproducible fabrication of MOREs with defined geometry and functionality.
  • Successful spectro-electrochemical detection of N, N, N, N'- Tetramethyl-p-phenyl-enediamine (TMPD) and its radical cation.
  • Optimization of UV-VIS spectroscopy capabilities by varying tip-to-substrate distance.

Conclusions:

  • The developed MOREs are suitable for SPECM applications.
  • MOREs facilitate electrochemical single live cell imaging.
  • Oxygen production in living algae (Eremosphaera viridis) was successfully detected using MOREs and local illumination.