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Related Experiment Video

Updated: Aug 28, 2025

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model
06:28

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Published on: January 6, 2011

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Transient Intraluminal Filament Middle Cerebral Artery Occlusion Stroke Model in Rats: A Step-by-Step Guide and

Konstantinos M Themistoklis1, Themistoklis I Papasilekas1, Konstantinos S Melanis2

  • 1Center of Basic Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece; Department of Neurosurgery, "Korgialenio, Benakio, HRC" General Hospital of Athens, Athens, Greece.

World Neurosurgery
|September 17, 2022
PubMed
Summary

The transient intraluminal filament middle cerebral artery occlusion (tifMCAO) rat model is a key tool for stroke research. This detailed guide refines the procedure, improving reproducibility and clinical relevance for stroke studies.

Keywords:
Cerebral ischemiaMiddle cerebral artery occlusionNeuroprotectionStrokeStroke modelsTransient ischemia

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Area of Science:

  • Neurology
  • Experimental Medicine
  • Surgical Models

Background:

  • Stroke is a major global health concern, driving significant research into pathophysiology and treatments.
  • Existing stroke models are crucial for laboratory research, yet require refinement for better clinical translation.
  • The transient intraluminal filament middle cerebral artery occlusion (tifMCAO) model is widely used for focal ischemia studies.

Purpose of the Study:

  • To provide a detailed, stepwise instructional guide for the transient intraluminal filament middle cerebral artery occlusion (tifMCAO) rat model.
  • To optimize the surgical technique for improved outcomes and reproducibility in focal stroke research.
  • To enhance the clinical relevance of the tifMCAO model for studying stroke and endovascular thrombectomy.

Main Methods:

  • The study details the surgical procedure for the tifMCAO model in Wistar rats (300-400 g).
  • Stepwise descriptions, accompanied by visual aids, guide the surgical technique, including arteriotomy of the external carotid artery stump.
  • The procedure was performed on 65 rats (male and female) across various experimental protocols.

Main Results:

  • Initial mortality rates of 48% were reduced to 10% with practice, demonstrating procedural optimization.
  • The mean procedural time was 53 minutes (range, 38-85 minutes).
  • A high stroke induction rate of 87.5% was achieved in a subset of 8 rats, confirming model efficacy.

Conclusions:

  • The tifMCAO model in rats is highly relevant for experimental focal ischemia research due to its clinical applicability.
  • This report offers a refined, 15-step instructional breakdown of the tifMCAO procedure for enhanced learning and application.
  • The optimized technique improves reproducibility and success rates, making it a valuable resource for stroke research laboratories.