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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
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Related Experiment Video

Updated: Aug 28, 2025

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
05:52

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

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Nanopore microscope identifies RNA isoforms with structural colours.

Filip Bošković1, Ulrich Felix Keyser2

  • 1Cavendish Laboratory, University of Cambridge, Cambridge, UK.

Nature Chemistry
|September 19, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces Amplification-free RNA TargEt Multiplex Isoform Sensing (ARTEMIS), a novel method for single-molecule RNA isoform identification. ARTEMIS bypasses enzymatic biases, enabling direct detection and quantification of RNA variants without amplification.

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Area of Science:

  • Molecular Biology
  • Nanotechnology
  • Genomics

Background:

  • Traditional RNA transcript isoform identification methods are complex and prone to enzymatic biases.
  • Accurate detection of RNA isoforms is crucial for understanding gene expression and disease mechanisms.

Purpose of the Study:

  • To develop a novel, amplification-free method for single-molecule RNA isoform identification and quantification.
  • To overcome the limitations of enzymatic biases in existing RNA analysis techniques.

Main Methods:

  • Design of three-dimensional molecular constructs (RNA identifiers) by refolding target RNA with complementary DNA strands.
  • Utilizing solid-state nanopore microscopy to read unique sequences of structural "colors" (DNA structures, protein labels, native RNA structures).
  • Implementing the Amplification-free RNA TargEt Multiplex Isoform Sensing (ARTEMIS) method for enzyme-free, single-molecule analysis.

Main Results:

  • ARTEMIS enables simultaneous identification and relative quantification of multiple RNA targets without prior amplification.
  • The method accurately reveals structural arrangements in native transcripts, consistent with published variants.
  • ARTEMIS successfully discriminates between circular and linear transcript isoforms in a complex human transcriptome in a single step.

Conclusions:

  • ARTEMIS provides a powerful, enzyme-free approach for high-resolution RNA isoform analysis at the single-molecule level.
  • This method offers a significant advancement for transcriptomics, overcoming amplification and enzymatic biases.
  • ARTEMIS has the potential to enhance the study of gene regulation and disease diagnostics.