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Anthocyanin Encapsulated Nanoparticles as a Pulmonary Delivery System
Madumani Amararathna1, David W Hoskin2, H P Vasantha Rupasinghe1,2
1Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS, Canada B2N 5E3.
Oxidative Medicine and Cellular Longevity
|September 20, 2022
Summary
Haskap berry anthocyanins encapsulated in carboxymethyl chitosan (CMC) nanocarriers show potential for direct lung delivery. These nanocarriers protect anthocyanins, enhance their therapeutic efficacy, and reduce lung tissue damage.
Area of Science:
- Pharmacology and Toxicology
- Materials Science
- Biotechnology
Background:
- Anthocyanins possess therapeutic properties but degrade after ingestion, limiting efficacy.
- Direct delivery systems can enhance anthocyanin stability, bioavailability, and therapeutic effects.
Purpose of the Study:
- To develop a direct delivery system for anthocyanins into pulmonary tissues using encapsulated nanocarriers.
- To evaluate the efficacy and safety of haskap berry anthocyanin-loaded carboxymethyl chitosan nanocarriers for lung delivery.
Main Methods:
- Haskap berry anthocyanins (cyanidin-3-O-glucoside rich) were encapsulated in polyethylene glycol-poly(lactide-co-glycolide), maltodextrin, and carboxymethyl chitosan (CMC) nanocarriers.
- Encapsulation efficiency, cytotoxicity, and cytoprotective effects were assessed in lung epithelial cells (BEAS-2B).
- In vivo studies in mice involved intranasal administration of nanocarriers, followed by UPLC/ESI/Q-TOF-MS analysis of lung tissue for anthocyanin detection.
Main Results:
- Carboxymethyl chitosan (CMC) exhibited significantly higher anthocyanin encapsulation efficiency compared to other polymers.
- CMC nanocarriers were non-cytotoxic to lung epithelial cells.
- Anthocyanin-loaded CMC nanoparticles reduced carcinogen-induced oxidative stress and DNA damage in lung cells.
- Anthocyanins and their metabolites were detected in mouse lung tissue after intranasal administration of nanocarriers.
Conclusions:
- Carboxymethyl chitosan is a promising non-cytotoxic material for encapsulating haskap berry anthocyanins for direct lung delivery.
- Direct delivery of anthocyanins to lung tissues via nanocarriers enhances retention, modulates metabolism, and improves therapeutic potential.
