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Updated: Aug 28, 2025
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Published on: June 9, 2017
Damage-free evaluation of cultured cells based on multivariate analysis with a single-polymer probe
Hiroka Sugai1,2, Shunsuke Tomita2, Sayaka Ishihara2
1Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan. sugai.hiroka.fn@u.tsukuba.ac.jp.
Abstract:
We present a pattern-recognition-based sensor that targets cell-derived components in culture media and evaluates cultured cells without damaging them. An array sensor made of a single-polymer probe was employed to obtain fluorescence response patterns of the analyte media, allowing successful identification of the type and state of the cells via multivariate analysis.
