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Comparison of RhD Typing Results by Serology and Molecular Methods
Richard R Gammon1, Michelle Conceicao2, Nancy Benitez3
1Scientific, Medical, Technical Direction, OneBlood, Orlando, FL, USA.
Objective:
Molecular testing determines D antigen status when abnormal serologic results are observed. Molecular testing is routinely batched, resulting in longer turnaround time for abnormal D status resolution. During the interim, obstetric patients with questionable/uninterpretable and weak D typing results by serology, per the immunohematology reference laboratory (IRL) policy, will receive RhD negative blood. This study aimed to determine whether serology results achieved a concordance.
Methods:
Six hospitals provided samples to the IRL (first IRL) for RhD status by DNA. De-identified samples were sent for serology RhD (second IRL). A concordance of ≥80% was acceptable.
Results:
Forty-nine samples were evaluated. Results were concordant (65.3% [32/49]) and discordant (34.7% [17/49]). This is significantly lower than clinically acceptable 80% (z = 2.57, P < .05). The turnaround-time was 3.0 hours for serology and 4.4 days for molecular evaluation.
Conclusion:
Due to a low concordance, serology could not be used in place of molecular testing.
