Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations
Dong Fu1,2
1Division of Pharmacotherapy and Experimental Therapeutics, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. dong.fu@fronteratherapeutics.com.
Methods in Molecular Biology (Clifton, N.J.)
|September 20, 2022
Abstract:
Collagen sandwich culture of hepatocytes retains many in vivo-like properties and is used for many investigations in liver cell biology, and hepatic pharmacology and toxicology. This chapter describes the method of establishing collagen sandwich culture of hepatocytes in a glass bottom dish for image-based studies.
Keywords:
Collagen sandwich cultureConfocal microscopeGlass bottom dishHepatocytesHepatotoxicityPolarizationPolarized trafficking

