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Updated: Aug 28, 2025

Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
Published on: June 14, 2024
Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis
Geraldy L S Liman1, Meghan E Stettler1, Thomas J Santangelo2
1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA.
Abstract:
Genetic manipulation is an essential tool to investigate complex microbiological phenomena. In this chapter we describe the techniques required to transform the model hyperthermophilic, anaerobic archaeon Thermococcus kodakarensis. T. kodakarensis can support two modes of genetic manipulation, dependent either on homologous recombination into the genome or through retention of autonomously replicating plasmids. The robust genetic system developed in T. kodakarensis offers a variety of selectable and counterselectable markers for complex, accurate and iterative genetic manipulations offering greater flexibility to probe gene function in vivo.
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