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Compositional differences of β-glucan-rich extracts from three relevant mushrooms obtained through a sequential
Zaida Pérez-Bassart1, Maria Jose Fabra2, Antonio Martínez-Abad2
1Food Safety and Preservation Department, Institute of Agrochemistry and Food Technology (IATA-CSIC), Valencia, Spain.
Food Chemistry
|September 20, 2022
Summary
This study analyzed mushroom biomass fractionation, revealing that β-glucan extraction varies by mushroom species. Grifola frondosa and Pleurotus ostreatus showed distinct β-glucan distribution, informing biomass valorization strategies.
Area of Science:
- * Food Science and Technology
- * Mycology
- * Biomass Valorization
Background:
- * Mushrooms are a significant global food source and a valuable source of bioactive compounds.
- * β-glucans, known for their health benefits, are key polysaccharides in mushroom cell walls.
- * Understanding polysaccharide extraction is crucial for maximizing mushroom biomass utilization.
Purpose of the Study:
- * To investigate the preferential extraction of compounds from three major mushroom species (Grifola frondosa, Lentinula edodes, Pleurotus ostreatus) using sequential fractionation.
- * To determine how mushroom source affects β-glucan extraction efficiency and localization.
- * To provide a basis for optimizing extraction processes for mushroom biomass valorization.
Main Methods:
- * Sequential fractionation using cold and hot aqueous and alkaline extractions.
- * Detailed compositional analysis including gross composition, monosaccharide analysis, Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).
- * Application of the protocol to Grifola frondosa, Lentinula edodes, and Pleurotus ostreatus.
Main Results:
- * Room temperature aqueous extracts yielded the highest overall amounts (56.3-82%), containing proteins, sugars, and polyphenols.
- * Pleurotus ostreatus exhibited the highest β-glucan content, concentrated in soluble fractions.
- * Grifola frondosa contained recalcitrant β-glucan primarily in the residue, while Lentinula edodes showed distributed β-glucan and higher chitin purity in the residue.
Conclusions:
- * The study demonstrates significant variations in β-glucan distribution and extractability among different mushroom species.
- * The findings highlight the potential for targeted extraction strategies to isolate specific compounds like β-glucans.
- * This research provides foundational data for the rational design of efficient mushroom biomass valorization processes.
