Colour-coded stereo images from the tandem scanning reflected light microscope (TSRLM)
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Updated: Aug 17, 2026

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Journal of Microscopy
|May 1, 1987
Abstract:
A method is described for the production of stereo-pair images in the tandem scanning reflected light microscope in which depths within the field imaged are coded in colour. The two images are recorded photographically whilst focusing vertically through the layer which is to be imaged. A horizontal component of motion is applied at the same time, but in opposing senses for the two images. Colour coding for depth is obtained by changing colour filters so that reflective features lying within different depth bands are imaged in corresponding colours.

