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Updated: Aug 12, 2026
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
Serological follow up of positive SARS-CoV-2 cases
José Gerardo González-González1, Adrián Camacho-Ortiz2, René Rodríguez-Gutiérrez3
1División de Endocrinología, Facultad de Medicina, Universidad Autónoma de Nuevo León and Hospital Universitario Dr. José E. González/Plataforma INVEST Medicina UANL-KER Unit Mayo Clinic (KER Unit Mexico). Monterrey, Nuevo Leon, Mexico. jgerardo@meduanl.com.
Objective:
To describe the humoral response in a cohort with mild and asymptomatic SARS-CoV-2 infection previ-ously identified in a community-based serological survey.
Materials And Methods:
This study was an observational follow up of 193 subjects previously identified with positive anti-SARS-CoV-2 antibodies invited for a second test 112 days after the first sampling. All completed a standardized electronic questionnaire. IgM/IgG antibodies were determined using a qualitative IgM/IgG chemiluminescent immunoassay.
Results:
Among the 193 eligible subjects, a total of 174 (90%) attended the follow-up visit, and their serum samples were tested. Of the samples, 171 (98.3%) were still positive, and 3 (1.7%) were negative. Also, the cut-off index (COI) value of the immunoassay significantly increased from the first to the second test (P <0.001).
Conclusions:
Our findings support a sustained humoral response in individuals with mild and asymptomatic SARS-CoV-2 infection up to 112 days after a positive serologic baseline test, accompanied by increasing antibody titers.
