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Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.0K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.0K
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

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Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
2.4K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
503
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

2.4K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
2.4K
Affinity Chromatography01:03

Affinity Chromatography

818
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Related Experiment Video

Updated: Aug 28, 2025

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
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Recent development and application of membrane chromatography.

Jing Chen1, Bing Yu1,2, Hailin Cong3,4

  • 1Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, China.

Analytical and Bioanalytical Chemistry
|September 21, 2022
PubMed
Summary

Membrane chromatography offers an effective alternative to traditional methods for protein and macromolecule purification. This review explores its development, applications in areas like virus purification and water treatment, and future potential.

Keywords:
Membrane chromatographyMembrane ligandMembrane modulesPurification

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Area of Science:

  • Biochemistry and Biotechnology
  • Separation Science
  • Environmental Engineering

Background:

  • Membrane chromatography is a key technique in downstream processing for separating and purifying proteins and biological macromolecules.
  • It serves as an efficient alternative to conventional column chromatography, offering improvements in affinity, hydrophobicity, and ion exchange.
  • Applications extend beyond bioprocessing to include areas like sewage disposal and water treatment.

Purpose of the Study:

  • To review the current development status of membrane chromatography, focusing on membrane matrix and equipment.
  • To summarize the diverse applications of membrane chromatography, including protein capture, virus purification, and water treatment.
  • To highlight the value of membrane chromatography for future exploration and application.

Main Methods:

  • Literature review of membrane chromatography development.
  • Analysis of membrane matrix and equipment advancements.
  • Compilation and summary of reported applications.

Main Results:

  • Membrane chromatography shows significant improvements over traditional chromatography in various aspects.
  • Established applications include protein purification, monoclonal antibody production, virus removal, and water treatment.
  • Ongoing developments in membrane materials and equipment are enhancing its capabilities.

Conclusions:

  • Membrane chromatography is a versatile and powerful separation technique with broad applicability.
  • Further advancements in its technology will likely expand its use in biopharmaceutical purification and environmental remediation.
  • This review provides a valuable resource for researchers and practitioners in the field.