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Related Experiment Video

Updated: Aug 28, 2025

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
08:28

Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers

Published on: September 4, 2017

10.0K

Microfluidic-assisted silk nanoparticle tuning.

Thidarat Wongpinyochit1, John D Totten1, Blair F Johnston1

  • 1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde 161 Cathedral Street Glasgow G4 0RE UK philipp.seib@strath.ac.uk.

Nanoscale Advances
|September 22, 2022
PubMed
Summary
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Microfluidics enables continuous production of silk nanoparticles with controlled characteristics. This method yields stable, non-cytotoxic nanoparticles suitable for biomedical applications, with size influencing cellular uptake.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Chemical Engineering

Background:

  • Silk is increasingly utilized in pharmaceutical and biomedical fields.
  • Silk can be processed into various formats, including nanoparticles, using different methods.
  • Controlling nanoparticle properties is crucial for effective biomedical applications.

Purpose of the Study:

  • To demonstrate the potential of microfluidics for continuous silk nanoparticle production.
  • To investigate the influence of microfluidic parameters on nanoparticle characteristics.
  • To assess the biocompatibility and cellular interactions of the produced silk nanoparticles.

Main Methods:

  • Utilized a microfluidic system for continuous mixing of aqueous silk solution with a solvent phase.

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Last Updated: Aug 28, 2025

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  • Controlled solvent ratios and flow rates to tune nanoparticle formation.
  • Characterized nanoparticle size, stability, and secondary structure (beta-sheet content).
  • Evaluated cytotoxicity using cell-based assays and assessed cellular trafficking.

    • Main Results:

    • Microfluidics allowed efficient, continuous production of silk nanoparticles.
    • Flow rate and solvent:aqueous ratios significantly impacted yield, size, and stability.
    • A solvent:aqueous ratio of 5:1 produced stable, spherical nanoparticles (110 and 215 nm) with high beta-sheet content.
    • Silk nanoparticles exhibited no cytotoxicity (IC50 > 100 μg mL⁻¹) and demonstrated size-dependent cellular trafficking.

    Conclusions:

    • Microfluidic-assisted manufacturing offers a controllable platform for producing silk nanoparticles.
    • Key processing variables in microfluidics can be manipulated to tune silk nanoparticle properties.
    • These tunable silk nanoparticles show promise for advanced pharmaceutical and biomedical applications.