Related Experiment Video
Updated: Aug 30, 2026

08:05
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
Published on: December 12, 2011
A comparison of three ELISA systems for the detection of Mycoplasma pulmonis antibody in rats
Abstract:
Barrier maintained and conventionally reared rats were tested for the presence of antibody to M. pulmonis using three ELISA test systems. Agreement between the two commercial test kits was 100% for both the barrier maintained and conventionally reared rats. Agreement between the kits and the non-commercial ELISA was 96% for barrier maintained rats, but only 56% for conventionally reared rats. Procedural differences or the presence of reactive substances in the sera of conventionally reared rats could have accounted for the discrepancy between the non-commercial and commercial tests.

