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Synthesis of Bioactive Silver Nanoparticles Using New Bacterial Strains from an Antarctic Consortium
Maria Sindhura John1,2, Joseph Amruthraj Nagoth1, Kesava Priyan Ramasamy1
1School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy.
Marine Drugs
|September 22, 2022
Summary
Three Antarctic marine bacteria were used to synthesize silver nanoparticles (AgNPs) within 24 hours. These novel AgNPs exhibit significant antimicrobial activity, offering a potential alternative to conventional antibiotics.
Area of Science:
- Microbiology
- Nanotechnology
- Biotechnology
Background:
- Novel bacterial strains were isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii.
- The synthesis of silver nanoparticles (AgNPs) using microbial agents is an area of growing interest for sustainable applications.
Purpose of the Study:
- To report the biosynthesis of silver nanoparticles (AgNPs) using three newly isolated bacterial strains: Rhodococcus, Brevundimonas, and Bacillus.
- To characterize the synthesized AgNPs and evaluate their antimicrobial properties.
Main Methods:
- Bacterial strains were incubated with a 1 mM silver nitrate (AgNO3) solution at 22 °C for 24 hours.
- UV-Vis spectroscopy was used to determine the maximum absorbance of the AgNPs.
- Energy-dispersive X-ray analysis (EDAX) and Transmission Electron Microscopy (TEM) were employed for characterization.
Main Results:
- AgNPs were successfully synthesized by Rhodococcus, Brevundimonas, and Bacillus strains.
- UV-Vis spectra showed maximum absorbances for AgNPs between 404 nm and 406 nm.
- TEM analysis revealed spherical and rod-shaped AgNPs (20-50 nm) with protein capping, and EDAX confirmed the presence of silver.
- The synthesized AgNPs demonstrated significant antimicrobial activity against common nosocomial pathogens.
Conclusions:
- The study successfully demonstrated the biosynthesis of AgNPs using novel bacterial strains from an Antarctic marine environment.
- The synthesized AgNPs possess antimicrobial properties, suggesting potential applications in medicine and environmental detoxification.
- This microbial synthesis offers a sustainable and eco-friendly approach to nanoparticle production.
