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Related Experiment Video

Updated: Aug 28, 2025

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Differentiation of Human Pluripotent Stem Cells into Insulin-Producing Islet Clusters

Published on: June 23, 2023

Differentiating functional human islet-like aggregates from pluripotent stem cells.

Tom Barsby¹, Hazem Ibrahim¹, Väinö Lithovius¹

¹Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland.

|September 22, 2022

Summary

This summary is machine-generated.

Researchers developed a 7-stage protocol to create human stem cell-derived pancreatic islets (SC-islets). These SC-islets mimic natural islets, showing glucose-sensitive insulin secretion for disease modeling and therapy.

Keywords:

Cell differentiation Stem cells

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Area of Science:

Stem cell biology
Endocrinology
Regenerative medicine

Background:

Diabetes mellitus requires functional pancreatic islets for glucose homeostasis.
Human pluripotent stem cells offer a potential source for generating insulin-producing beta cells.
Existing protocols for stem cell-derived islet generation often lack robustness and efficiency.

Purpose of the Study:

To establish a reliable and robust protocol for differentiating human pluripotent stem cells into pancreatic islet-like aggregates (SC-islets).
To characterize the functional properties and cellular composition of the generated SC-islets.
To assess the suitability of SC-islets for in vitro disease modeling and therapeutic applications.

Main Methods:

A 7-stage differentiation protocol was employed, mimicking developmental patterning factors.
The protocol integrated monolayer, microwell, and aggregate suspension culture techniques.
SC-islets were evaluated for glucose-sensitive insulin secretion and endocrine cell composition.

Main Results:

The protocol successfully generated pancreatic islet-like aggregates (SC-islets) from human pluripotent stem cells.
SC-islets exhibited dynamic, glucose-sensitive insulin secretion.
The endocrine cell composition of SC-islets closely resembled that of primary human islets.

Conclusions:

The presented protocol provides a robust and reliable method for generating functional SC-islets.
SC-islets are suitable for in vitro modeling of islet cell pathophysiology.
These SC-islets hold promise for future therapeutic applications in diabetes treatment.