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Related Concept Videos

Cell Lines01:16

Cell Lines

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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Human Primary Odontoblast-like Cell Cultures-A Focused Review Regarding Cell Characterization.

Christian Klein1,2, Christian Meller2, Edgar Schäfer3

  • 1HealthCare Center Meller Zahngesundheit Schlauzahn MVZ GmbH, D-71332 Waiblingen, Germany.

Journal of Clinical Medicine
|September 23, 2022
PubMed
Summary

Establishing reliable odontoblast-like cell cultures is challenging due to a lack of standardized methods for isolation and characterization. Current models often fail to definitively confirm an odontoblast-like nature, hindering their use in research.

Keywords:
cell culturecharacterizationodontoblast-likeosteoblast-likestem cell

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Dental Research

Background:

  • Odontoblasts, crucial for dentin formation, are postmitotic and difficult to culture.
  • Existing "odontoblast-like" cell culture models lack standardized protocols for isolation, induction, and characterization.
  • There is a critical need for validated in vitro models to study dentin biology.

Purpose of the Study:

  • To critically evaluate methods for establishing "odontoblast-like" cell cultures.
  • To assess the validity of molecular techniques used for characterizing these cell models.
  • To identify gaps in current research regarding the standardization and quality control of odontoblast-like cell cultures.

Main Methods:

  • Systematic literature review of studies published between 2016 and 2022.
  • Analysis of data on cell culture conditions, stem cell screening, induction media, mineralization, and cell characterization.
  • Searches conducted in Medline, Scopus, and Web of Science databases.

Main Results:

  • Included studies failed to provide definitive confirmation of an "odontoblast-like" nature.
  • Characterization methods often did not adequately distinguish cells from bone, despite developmental similarities.
  • Lack of standardized protocols and quality control measures was evident across reviewed publications.

Conclusions:

  • Current "odontoblast-like" cell culture models require significant improvement in standardization and validation.
  • Robust characterization must demonstrate similarity to dentin and clear distinction from bone.
  • Further research is needed to develop reliable and reproducible in vitro models for odontoblast research.