Cystitis Induces Altered CREB Expression Related with Micturition Reflex
1Department of Urology, School of Medicine, Kyung Hee University, Seoul 05278, Korea.
Medicina (Kaunas, Lithuania)
|September 23, 2022
Summary
Bacterial bladder infections, like E. coli cystitis, alter neurotrophin signaling. Chronic infections significantly increase phosphorylated-CREB (p-CREB) in rat dorsal root ganglia, indicating pathological changes.
Area of Science:
- Neuroscience
- Urology
- Molecular Biology
Background:
- Bladder stimulation influences neurotrophins crucial for the voiding reflex.
- Bacterial cystitis can activate this system, leading to pathological states.
- Phosphorylated-CREB (p-CREB) is a key transcription factor in neurotrophin signaling.
Purpose of the Study:
- To investigate changes in p-CREB expression within rat dorsal root ganglia (DRG) following uropathogenic Escherichia coli infection.
Main Methods:
- Adult female Sprague-Dawley rats were subjected to acute E. coli infection, chronic E. coli infection, or served as controls.
- p-CREB immunoreactivity (p-CREB-IR) was quantified in lumbosacral DRG (L1–L6, S1) sections.
- Cells with intense nuclear staining were identified as positive for p-CREB-IR.
Main Results:
- Chronic E. coli cystitis significantly elevated p-CREB-IR in L1–L6 and S1 DRG compared to controls (p < 0.05).
- Chronic cystitis showed significantly higher p-CREB-IR in L3–L6 and S1 DRG than acute cystitis (p < 0.05).
- p-CREB-IR was lower in L4–L5 DRG in control and acute cystitis groups compared to other DRG sections (p < 0.05).
Conclusions:
- Both acute and chronic E. coli cystitis alter p-CREB immunoreactivity in lumbosacral DRG cells.
- Chronic E. coli infection leads to p-CREB overexpression in specific DRG regions (L1–L6, S1).
- This overexpression suggests the development of subsequent pathological changes associated with chronic cystitis.
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