Research on Dual-Technology Fusion Biosensor Chip Based on RNA Virus Medical Detection

Area of Science:

• Nanotechnology
• Biosensing
• Virology

Background:

• Emerging RNA viruses like COVID-19 necessitate advanced public health emergency responses.
• Existing detection methods face limitations in sensitivity and accuracy for rapid screening.

Purpose of the Study:

• To design and simulate an electrodeless biosensing chip for enhanced RNA virus detection.
• To investigate the impact of gold nanorod configurations on local surface plasmon resonance (LSPR) and biosensor performance.
• To evaluate the advantages of combining quartz crystal microbalance (QCM) and LSPR technologies in an electrode-free format.

Main Methods:

• Utilized COMSOL finite element simulation software to analyze plasmonic resonance in gold nanorod-quartz substrates.
• Simulated surface potential distribution of the QCM biosensing chip with and without electrodes.
• Investigated the effect of gold nanorod dimer arrangement and spacing on LSPR characteristics.

Main Results:

• Gold nanorod dimer arrangement significantly influences LSPR and biomolecule detection outcomes.
• High-concentration "hot spots" observed in gold nanorod dimers indicate strong resonance mode hybridization.
• An electrodeless biosensor design demonstrated superior sensing performance compared to traditional electrode-based chips.
• The combined QCM and LSPR approach reduces metal electrode interference, improving detection sensitivity and accuracy.

Conclusions:

• An electrodeless biosensor integrating QCM and LSPR technologies offers a promising platform for RNA virus detection and screening.
• Optimized gold nanorod dimer structures are crucial for maximizing LSPR-based signal enhancement.
• The electrode-free design significantly improves biosensor sensitivity and accuracy, addressing limitations of conventional methods.